Cloned (Comment) | Organism |
---|---|
gene ccl or PA0883 , genetic structure, the enzyme is encoded in a six-gene operon | Pseudomonas aeruginosa |
gene ccl or Y2383 or ripC, genetic structure, the enzyme is encoded in a three-gene operon, in vitro reconstitution of itaconate degradation pathway with the heterologously produced and purified enzymes from Yersinia pestis | Yersinia pestis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Pseudomonas aeruginosa | |
Mg2+ | activates | Yersinia pestis | |
Mg2+ | activates | Pseudomonas aeruginosa | |
Mn2+ | activates | Yersinia pestis | |
Mn2+ | activates | Pseudomonas aeruginosa | |
additional information | activity of YpCcl is dependent on the presence of divalent cations such as Mg2+ or Mn2+ | Yersinia pestis | |
additional information | the enzyme PaCcl requires divalent metal ions, such as Mg2+, Mn2+ or Co2+, for activity | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-citramalyl-CoA | Yersinia pestis | - |
acetyl-CoA + pyruvate | - |
? | |
(S)-citramalyl-CoA | Pseudomonas aeruginosa | - |
acetyl-CoA + pyruvate | - |
? | |
(S)-citramalyl-CoA | Yersinia pestis Y2384 | - |
acetyl-CoA + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I562 | gene ccl or PA0883 | - |
Yersinia pestis | - |
gene ccl or Y2383 or ripC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-citramalyl-CoA | - |
Yersinia pestis | acetyl-CoA + pyruvate | - |
? | |
(S)-citramalyl-CoA | - |
Pseudomonas aeruginosa | acetyl-CoA + pyruvate | - |
? | |
(S)-citramalyl-CoA | - |
Yersinia pestis Y2384 | acetyl-CoA + pyruvate | - |
? | |
additional information | PaCcl is highly specific for its substrate, (S)-citramalyl-CoA. It also catalyzes stereospecifically the (R)-3-hydroxy-3-methylglutaryl-CoA lyase reaction and the (3S)-citryl-CoA lyase reaction, EC 4.1.3.34, albeit with much lower catalytic efficiency. No activity with (S)-3-hydroxy-3-methylglutaryl-CoA | Pseudomonas aeruginosa | ? | - |
? | |
additional information | YpCcl does not accept other tested beta-hydroxyacyl-CoA compounds, such as 3-hydroxy-3-methylglutaryl-CoA, (3S)-malyl-CoA or beta-methylmalyl-CoA as substrates, but it catalyzes the (3S)-citryl-CoA lyase reaction, EC 4.1.3.34, albeit with lower catalytic efficiency | Yersinia pestis | ? | - |
? | |
additional information | YpCcl does not accept other tested beta-hydroxyacyl-CoA compounds, such as 3-hydroxy-3-methylglutaryl-CoA, (3S)-malyl-CoA or beta-methylmalyl-CoA as substrates, but it catalyzes the (3S)-citryl-CoA lyase reaction, EC 4.1.3.34, albeit with lower catalytic efficiency | Yersinia pestis Y2384 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCL | - |
Yersinia pestis |
CCL | - |
Pseudomonas aeruginosa |
CitE-like protein | - |
Yersinia pestis |
CitE-like protein | - |
Pseudomonas aeruginosa |
YpCcl | - |
Yersinia pestis |
General Information | Comment | Organism |
---|---|---|
evolution | the itaconate degradation and detoxification pathways of Yersinia and Pseudomonas, both possessing three genes for itaconate degradation, i.e. itaconate coenzyme A (CoA) transferase, itaconyl-CoA hydratase and (S)-citramalyl-CoA lyase, encoded in the rip operon, are the result of convergent evolution | Yersinia pestis |
evolution | the itaconate degradation and detoxification pathways of Yersinia and Pseudomonas, both possessing three genes for itaconate degradation, i.e. itaconate coenzyme A (CoA) transferase, itaconyl-CoA hydratase and (S)-citramalyl-CoA lyase, encoded in the rip operon, are the result of convergent evolution | Pseudomonas aeruginosa |
metabolism | the enzyme catalyzes the last step of the itaconate degradation pathway | Yersinia pestis |
metabolism | the enzyme catalyzes the last step of the itaconate degradation pathway | Pseudomonas aeruginosa |
physiological function | the enzyme is crucial for survival of the pathogen in host macrophages | Yersinia pestis |
physiological function | the enzyme is crucial for survival of the pathogen in host macrophages | Pseudomonas aeruginosa |